Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9236666 | Clinical Immunology | 2005 | 6 Pages |
Abstract
A novel cDNA cloning strategy consisting in elimination of non-coding DNA sequences from 3â² regions of cDNAs was applied to construct the Taenia crassiceps phage displayed cDNA expression library. After biopanning using immune sera, three phage clones expressing T. crassiceps-derived antigens specifically recognizing antibodies present in cerebrospinal fluid and plasma samples from neuroimaging-confirmed neurocysticercosis patients were selected. This novel cloning strategy may be applied to other pathogens allowing rapid identification of peptides/proteins for immunodiagnostic tests.
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Authors
Yetzi Robles, Erik González, Tzipe Govezensky, Maria Elena Mungia, Gonzalo Acero, Raul J. Bobes, Goar Gevorkian, Karen Manoutcharian,