Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9236734 | Clinical Immunology | 2005 | 11 Pages |
Abstract
We have shown that tacrolimus (TAC)-induced liver allograft acceptance is associated with migration and persistence of donor B cells and dendritic cells (DC). To clarify whether these MHC class II+ leukocytes have favorable roles in inducing tolerance, we analyzed recipient T cell reactions after allogeneic B or DC infusion. LEW rat B cells localized exclusively in BN host B cell follicles without any direct contact with host T cells. While few donor DC migrated to T cell areas and marginal zones, they were captured by host APC, suggesting that allogeneic MHC class II+ cells may induce immune reactions via the indirect pathway. Although DC-infused non-immunosuppressed recipients showed enhanced ex vivo anti-donor responses, persistent in vitro donor-specific hyporeactivity was seen equally with donor DC or B cell infusion under TAC. The results indicate that donor MHC class II+ APC are capable of regulating recipient immune reactions under TAC. Possible involvement of the indirect pathway of allorecognition is discussed.
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Authors
Olga Azhipa, Kei Kimizuka, Atsunori Nakao, Hideyoshi Toyokawa, Toyokazu Okuda, Joao Seda Neto, Sean M. Alber, Takashi Kaizu, Angus W. Thomson, Anthony J. Demetris, Noriko Murase,