Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9245178 | Gastroenterology | 2005 | 14 Pages |
Abstract
Background & Aims: Despite the proven ability of immunization to prevent Helicobacter infection in mouse models, the precise mechanism of protection has remained elusive. Methods: We explored the cellular events associated with Helicobacter clearance from the stomach following vaccination by flow cytometry analysis and histological and molecular studies. Results: Kinetic studies showed that the infection is undetectable in vaccinated mice at day 5 postbacterial challenge. Flow cytometry analysis showed that the percentages of mast cells (CD3âCD117+) increased in the lymphoid cells isolated from the stomach at day 4 postchallenge in urease + cholera toxin (CT)-vaccinated mice in comparison with mice administered with CT alone (9.4% ± 4.4% and 3.1% ± 1%, respectively, for vaccinated and CT administered, n = 5; P < .01). Quantitative PCR analysis showed an increased messenger RNA (mRNA) expression of the mast cell proteases 1 and 2 at day 5 postchallenge in the stomach of vaccinated mice. In contrast to wild-type mice, mast cell-deficient mice (W/Wv mice) were not protected from H felis colonization after vaccination. Indeed only 1 out of 12 vaccinated W/Wv mice showed a negative urease test. Remarkably, vaccinated W/Wv mice reconstituted with cultured bone marrow-derived mast cells recovered the ability to clear the infection after vaccination (8 out of 10 mast cell-reconstituted mice showed negative urease tests [P < .006 as compared with wild-type mice]). Conclusions: These experiments show that mast cells are, unexpectedly, critical mediators of anti-Helicobacter vaccination.
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Authors
Dominique Velin, Daniel Bachmann, Hanifa Bouzourene, Pierre Michetti,