| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9258884 | Transfusion and Apheresis Science | 2005 | 10 Pages |
Abstract
As neutrophil activation is related to several adverse transfusion reactions, we studied about the activation induced by anti-neutrophil antibodies and the stabilizing effects of albumin pretreatment by means of flow cytometry. Anti-neutrophil monoclonal antibody (anti-HNA-1a, 1b, 2a) alone induced CD11b expression and shedding of L-selectin, and anti-HNA-1a reinforced reactive oxygen species (ROS) production. Albumin pretreatment significantly reduced CD11b expression and L-selectin shedding induced by fMLP and ROS production induced by PMA, G-CSF combined with PMA or LPS-fMLP, or anti-HNA-1a combined with PMA. These findings suggest that anti-HNA-1a is related to adverse reactions and albumin has a regulating effect on neutrophil activation.
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Authors
Makoto Hashimoto, Katsuyasu Saigo, Yukie Jyokei, Manami Kishimoto, Mariko Takenokuchi, Nobuo Araki, Shion Imoto, Kikuyo Taniguchi, Shunichi Kumagai,