Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9263326 | Diagnostic Microbiology and Infectious Disease | 2005 | 6 Pages |
Abstract
We characterized seven isolates of imipenem-resistant Achromobacter xylosoxidans that were isolated from patients hospitalized in the intensive care unit at a tertiary hospital in Korea during 2001 to 2003. From the analysis with an isoelectric focusing, polymerase chain reaction, and sequencing methods, all isolates were found to produce VIM-2, OXA-30, and chromosomal AmpC β-lactamase with a pI of 8.4. They showed a similar antibiogram, which were resistant to all tested aminoglycosides as well as β-lactams including imipenem (16-32 mg/L) and aztreonam (128 mg/L), and a same DNA fingerprinting pattern by random amplified polymorphic DNA analysis, suggesting that these originated from a single clone. From the analysis of integron structure carried by an isolate of A. xylosoxidans CBU1760, blaVIM-2 was found to be part of a gene cassette carried on a class 1 integron (3.4 kb) containing three aacA4 gene cassettes. This is the first report of blaVIM-2 in A. xylosoxidans.
Related Topics
Life Sciences
Immunology and Microbiology
Applied Microbiology and Biotechnology
Authors
Kyeong Seob Shin, Kyudong Han, Jungnam Lee, Seung Bok Hong, Bo Ra Son, Sei Jin Youn, Jungmin Kim, Hyung Sik Shin,