Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9264492 | Human Immunology | 2005 | 5 Pages |
Abstract
The aim of this study was to assess the possible association of the functional (GT)n microsatellite polymorphism in the FOXP3 gene with predisposition to several autoimmune diseases such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), ulcerative colitis (UC), Crohn's disease, and celiac disease. We analyzed a case-control cohort composed of 231 SLE patients, 293 RA patients, 528 inflammatory bowel disease (354 Crohn's disease patients and 260 UC patients) patients, 103 celiac disease patients, and 274 healthy controls ethnically matched. Genotyping of (GT)n microsatellite was performed by polymerase chain reaction (PCR)-based method combined with fluorescent technology. We found no evidence for association of this polymorphism between controls and these autoimmune disease patients. Additionally, no differences in the genotype and allele distribution were found when patients were stratified according to clinical manifestation. The (GT)n microsatellite of the FOXP3 gene may not play a relevant role in the susceptibility to SLE, RA, inflammatory bowel disease, and celiac disease in our population.
Keywords
FOXP3 geneTregsPBMCT1DIBDRheumatoid arthritis (RA)Rheumatoid arthritisHuman leukocyte antigenHLAAutoimmune diseasesCeliac diseaseCrohn’s diseaseInflammatory bowel diseaseType 1 diabetesMicrosatellitePeripheral blood mononuclear cellRegulatory T cellsconfidence intervalSystemic lupus erythematosusSystemic lupus erythematosus (SLE)SLEodds ratiopolymerase chain reactionPCRAIDUlcerative colitisUlcerative colitis (UC)
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Authors
Elena Sánchez, Blanca Rueda, Gisela Orozco, Javier Oliver, Jose R. Vilchez, Laura Paco, Miguel A. López-Nevot, José L. Callejas, José M. Sabio, Maria Gómez-Garcia, A. Nieto, Mario Delgado, Javier MartÃn,