Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9266213 | Immunology Letters | 2005 | 7 Pages |
Abstract
To investigate the effect of stress on interferon (IFN)-γ production by intestinal intraepithelial lymphocytes (IEL), we exposed male C3H/HeN mice to electric foot shock for 30 min a day for 5 consecutive days. Immediately after the final foot shock stress, IEL from small intestine were isolated by Percoll density gradient. The stress induced a marked suppression of IFN-γ production by IEL stimulated with immobilized anti-CD3 mAb and a marked decrease in the proportion of IFN-γ-producing CD3+ IEL or αβTCR+ IEL stimulated with PMA + ionomycin. The αβTCR+ subset was the major cause of stress-induced suppression of IFN-γ production by IEL. Glucocorticoid induced the suppression of IFN-γ production by IEL in vitro, which was reversed by mifepristone (RU486), a glucocorticoid receptor antagonist. In vivo administration of RU486 reversed the stress-induced suppression of IFN-γ production by IEL. In conclusion, repeated foot shock stress suppressed IFN-γ production of IEL by stress-induced elevation of endogenous glucocorticoid. Substantial suppression of the αβTCR+ subset was the major cause of the suppression.
Related Topics
Life Sciences
Immunology and Microbiology
Immunology
Authors
Xiumin Zhang, Mitsuharu Okutsu, Osamu Kanemi, Ryoichi Nagatomi,