Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9269229 | Journal of Hospital Infection | 2005 | 9 Pages |
Abstract
A new super-oxidized water (SOW) product, Microcyntm, was tested for in vitro antimicrobial and antiviral activities. The effectiveness of this neutral-pH SOW at killing Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and Candida albicans in pure culture was evaluated. One millilitre (â¼108Â colony-forming units/mL) of each micro-organism was subjected to 9Â mL Microcyn or sterile water at room temperature for 30Â s. Under these conditions, a log10 reduction factor of 8 in the level of all pathogens occurred in the treatment samples. In addition, results of tests with three batches of Microcyn exposed to Bacillus atrophaeus spores for 5Â min demonstrated complete inactivation of the spores within 2-3Â min (log10 reduction factor >4). The effectiveness of Microcyn in reducing human immunodeficiency virus-1 (HIV-1) on hard surfaces (glass) was also evaluated in compliance with Environmental Protection Agency requirements for virucidal claims. After exposure of the tested surfaces to Microcyn for 5Â min without agitation, there was a log10 reduction factor >3 in the viral load as measured by both cytopathic effect and antigen p24 of HIV-1 production in MT-2 cultures. Microcyn activity against adenoviral vector type 5 was also analysed under simulated laboratory in-use conditions with viral suspensions. In order to increase the sensitivity of the test, the fluorescent light emitted by AdGFP-infected cells was measured with the use of a flow cytometer. A log10 reduction factor >3 in the viral load was achieved after a 5-min exposure to Microcyn under these strict conditions. These results show that Microcyn exerts a wide antimicrobial spectrum with major advantages over acidic SOWs, including neutral pH, lower free active chlorine (51-85Â ppm) and long shelf life (1 year).
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Authors
C. Landa-Solis, D. González-Espinosa, B. Guzmán-Soriano, M. Snyder, G. Reyes-Terán, K. Torres, A.A. Gutierrez,