Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9278876 | FEMS Yeast Research | 2005 | 7 Pages |
Abstract
We previously have reported that Saccharomyces cerevisiae mutants expressing Kllsm4Î1, a truncated form of the KlLSM4 gene, as well as mutants in genes of the mRNA-decapping pathway, show phenotypic markers of apoptosis, increased temperature sensitivity and reduced growth in the presence of different drugs and oxidative stressing agents, such as acetic acid and H2O2. To isolate multicopy extra-genic suppressors of these defects, we transformed the Kllsm4Î1 mutant with a yeast DNA library and we selected a series of clones showing resistance to acetic acid. One of these clones carried a DNA fragment containing the HIR1 gene that encodes a transcriptional co-repressor of histone genes. The over-expression of HIR1 in the Kllsm4Î1 mutant prevented rapid cell death during chronological aging, reduced nuclei fragmentation and increased resistance to H2O2. Transcription analysis revealed that the expression of histone genes was lowered in the mutant over-expressing HIR1, indicating a relationship between the latter gene and apoptosis.
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Authors
Cristina Mazzoni, Vanessa Palermo, Mirko Torella, Claudio Falcone,