Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9279413 | Journal of Virological Methods | 2005 | 7 Pages |
Abstract
Genotypic assays are used often to guide clinicians in decisions concerning the treatment of patients. An optimized sequence-based genotypic assay was used to determine the whole protease and reverse transcriptase (RT) gene, including the gag cleavage site region and RNase H region. Since non-B subtypes are increasing in countries where subtype B was the most prevalent subtype, and treatment becomes more available in developing countries where the epidemic is characterized by a high prevalence of non-B subtypes, it was important that the genotypic test was evaluated using a panel of different subtypes. Amplification was successful for different subtypes: A, B, C, D, F, G, H, J, CRF01_AE, CRF02_AG, CRF11_cpx, CRF13_cpx and an uncharacterized recombinant sample. The detection limit of the PCR was 1000Â copies/ml, except for 1 subtype C sample (PL3) and 1 CRF02_AG sample (PL8). The detection limit for these samples was 5000Â copies/ml. A sequence could be obtained in both directions for most of the samples.
Keywords
Related Topics
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Immunology and Microbiology
Virology
Authors
J. Snoeck, C. Riva, K. Steegen, Y. Schrooten, B. Maes, L. Vergne, K. Van Laethem, M. Peeters, A.-M. Vandamme,