| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9279451 | Journal of Virological Methods | 2005 | 7 Pages |
Abstract
A fluorescence-based assay is presented for measuring the cytoxicity of viral proteins added exogenously to cells. The assay is based on the use of two fluorescent dyes, calcein-AM and ethidium homodimer (EtD-1) to specifically stain living and dead cells respectively and employs fluorescence activated cells sorting (FACS) to achieve a rapid and accurate measurement of the cytotoxic capacity of a potential viral toxin. The assay has been developed using the group B homologue (ADRV-NSP4) of the NSP4 enterotoxin encoded by Group A rotaviruses but should be applicable to assaying any viral protein exhibiting cytotoxic activity.
Keywords
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Authors
E. Guzman, M.A. McCrae,