| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9279511 | Journal of Virological Methods | 2005 | 5 Pages |
Abstract
A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.
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Authors
P. Saldarelli, H. Keller, M. Dell'Orco, A. Schots, V. Elicio, A. Minafra,