Article ID Journal Published Year Pages File Type
9288148 Virology 2005 11 Pages PDF
Abstract
Vif is dispensable for simian immunodeficiency virus (SIV) replication in some cells, termed permissive (i.e., CEM-SS), but not in others, termed non-permissive (i.e., H9, CEMx174, and peripheral blood lymphocytes). Non-permissive cells express the RNA editing enzyme, APOBEC3G. To determine whether vif mRNA could be alternatively spliced, a mutation altering the putative vif splice acceptor site (SA1) was introduced into SIVmac239 (SIVΔvif-SA). Despite three consensus splice acceptor sites nearby SA1, SIVΔvif-SA did not efficiently generate alternatively spliced vif mRNA. SIVΔvif-SA was growth attenuated in CEMx174 and H9 cells but not in CEM-SS cells. Following SIVΔvif-SA, but not SIVmac239, infection in either H9 or CEMx174 cells viral cDNA contained numerous G to A mutations; no such differences were observed in CEM-SS cells. This pattern is consistent with mutations generated by APOBEC3G in the absence of Vif. Therefore, efficient splicing of SIV vif mRNA is tightly controlled and requires the SA1 site.
Related Topics
Life Sciences Immunology and Microbiology Virology
Authors
, ,