Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9289258 | Virus Research | 2005 | 15 Pages |
Abstract
Certain neurotropic viruses can invade the nervous system of their hosts and spread in chains of synaptically connected neurons. Consequently, it is possible to identify entire hierarchically connected circuits within an animal. In this review, we discuss the use of neurotropic herpesviruses as neuronal tract tracers. Although a variety of tract tracing viruses are available, each with its own unique infection characteristics, we focus on the widespread use of attenuated strains of pseudorabies virus (PRV), a swine herpesvirus with a broad host range. In particular, we focus on new applications of PRV for tract tracing including use of multiple infections by PRV reporter viruses to test for circuit convergence/divergence within the same animal. We provide examples of these combined application techniques within the context of an animal model to study the naturally occurring reversal of seasonal obesity in Siberian hamsters.
Keywords
6-OHDAPSNsPCMV2-Deoxy-glucoseCT-BIWATMELCTFIBATmRFPPHA-LAVPN-methyl-d-aspartateNMDANPY6-HydroxydopamineGFPBATMEA2DGMPORNAimedial amygdalainguinal white adipose tissuebrown adipose tissueInterscapular brown adipose tissueMTIIParasympathetic nervous systemperipheral nervous systemPVNMelatoninMelanotan IIcentral tegmental fieldnorepinephrineparaventricular hypothalamic nucleusmedial preoptic nucleusanterior hypothalamusarginine vasopressingreen fluorescent proteinmonomeric red fluorescent proteinCytomegalovirus promoteroptic chiasmPNScholera toxin B subunitEstrogen receptorNeuropeptide Y
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Authors
C. Kay Song, Lynn W. Enquist, Timothy J. Bartness,