Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9289274 | Virus Research | 2005 | 7 Pages |
Abstract
The Tomato spotted wilt virus ambisense M- and S-RNA segments contain an A/U-rich intergenic region predicted to form a stable hairpin structure. The site of transcription termination of S-segment encoded N and NSs mRNAs synthesised in an in vitro transcription system was roughly mapped to the 3â²-end of the intergenic hairpin, i.e. position 1568-1574 for N and position 1852-1839 for NSs, as determined by RT-PCR cloning and size estimation on Northern blots. This suggests that these viral transcripts contain a predicted stem-loop structure at their 3â²-end. The potential involvement of the 3â²-end structure in transcription termination is discussed.
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Authors
Ingeborg van Knippenberg, Rob Goldbach, Richard Kormelink,