Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9289418 | Virus Research | 2005 | 5 Pages |
Abstract
The complete nucleotide sequence of foot-and-mouth disease virus (FMDV) O/FRA/1/2001 (bovine isolate) was determined from five cDNA clones covering most of the genome and compared with the British porcine isolate (O/UKG/35/2001) it originated from. Seven substitutions, out of which three resulted in amino acid changes (in the leader protease, 3A protein and 3D RNA-dependent RNA polymerase sequences) were identified and confirmed by direct sequencing of RT-PCR products obtained from in vitro infected cells and skin vesicles of an infected cow. RACE amplification allowed determination of the exact 3â² end of the genome. These changes and possibly the unusual 3A substitution between the British and its French derivate may account for the consecutive species shifts.
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Authors
Isabelle Nobiron, Michelle Rémond, Claude Kaiser, Françoise Lebreton, Stéphan Zientara, Bernard Delmas,