Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9289472 | Virus Research | 2005 | 4 Pages |
Abstract
Xenopus laevis oocytes can be used as an alternative system to study replication of Theiler's murine encephalomyelitis virus (TMEV). We have shown that transcript RNA, containing full-length viral genome, can be directly used to programme the oocytes. In the programmed oocytes, there is correct viral translation, polyprotein processing and assembly of capsid proteins leading to the production of infectious TMEV. The vast majority of de novo synthesised virions were found in the medium in which the programmed oocytes were incubated and not in the oocytes.
Keywords
Related Topics
Life Sciences
Immunology and Microbiology
Virology
Authors
David Franco, Ilse Stevens, Stephane Steurbaut, Jozef Goris, Bart Rombaut,