Vitrification of mouse pronuclear embryos after polar body biopsy without direct contact with liquid nitrogen

Cryopreservation of biopsied mouse pronuclear embryos in open-pulled straws, which are placed inside a hermetically closed container, guarantees a complete isolation of embryos from liquid nitrogen and avoids potential contamination by pathogenic microorganisms. The combination of plunging this container into liquid nitrogen and rapid warming makes this process as efficient as conventional vitrification.