Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9410612 | Molecular Brain Research | 2005 | 4 Pages |
Abstract
We previously demonstrated that the brain, pituitary, and C6 glioblastoma cells express leptin. To determine the physiological role of brain-derived leptin, we sought to selectively silence its expression using RNA interference (RNAi) in vitro. One of four potential targets, siRNA L7, reduced leptin mRNA by 50% (PÂ <Â 0.05) and protein by 55% (PÂ <Â 0.0001) in C6 cells. RNAi also induced a twofold increase in cell death as seen by ethidium homodimer-1 (PÂ <Â 0.015) and TUNEL (PÂ <Â 0.005) staining. These data suggest that endogenous leptin may be a critical factor promoting cell survival in the brain.
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Authors
Russell Brown, Barbara Morash, Ehud Ur, Michael Wilkinson,