Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9410927 | Molecular Brain Research | 2005 | 6 Pages |
Abstract
In situ hybridization can be carried out using different methods. The experimenter has to choose various parameters: the type of tissue fixation, the time of incubation, and the duration of the exposure time. All these parameters are determinant for the sensitivity and the resolution of this technique. This publication of technical aspects described different experiments performed for in situ hybridization on liver tissue. We may conclude on the parameters to optimize each step of the hybridization procedure. Moreover, this technique could be transposed to the brain and applied to little structures with a light expression of DHAP-AT.
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Cellular and Molecular Neuroscience
Authors
Agnès André, Christian Tessier, Lionel Brétillon, Jean-Louis Sébédio, Jean-Michel Chardigny,