Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9415680 | Brain Research | 2005 | 7 Pages |
Abstract
Vagotomy and capsaicin treatment attenuate dorsal vagal complex (DVC) but not myenteric Fos-like immunoreactivity (Fos-LI) induced by cholecystokinin-8 (CCK-8). The goal of this experiment is to test the role of the sympathetic nervous system in the pathway by which CCK-8 increases myenteric Fos-LI. Adult male Sprague-Dawley rats were pretreated with guanethidine sulfate (40 mg/kg daily for 5 weeks) or vehicle intraperitoneally (IP), and injected with CCK-8 (40 μg/kg) or saline IP. Fos-LI was then quantified in the DVC and the myenteric neurons of the duodenum and jejunum using a diaminobenzidine reaction. Guanethidine pretreatment attenuated myenteric but not DVC Fos-LI induced by CCK-8. These findings demonstrate that sympathetic neurons play a role in mediating the myenteric Fos-LI response to CCK. They also suggest differential mediation of myenteric and DVC responses to CCK.
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Authors
Stephen Gulley, Shannon J. Raboin, Sheryce C. Henley, Wai-Chu Chan, Sanjay K. Sharma, Leonard H. Billups, Alton R. Esdaile, Cherese N. Sullivan, Timothy H. Moran, Ayman I. Sayegh,