Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9415908 | Brain Research | 2005 | 8 Pages |
Abstract
The present study is designed to investigate the effects of preconditioning with different doses of hydrogen peroxide (H2O2) on oxidative stress-induced apoptosis and the changes in mitochondrial membrane potential (MMP), intracellular reactive oxygen species (ROS) level, and expression of Bcl-2 during H2O2 preconditioning in rat pheochromocytoma (PC12) cells. It was shown that (1) H2O2 induced apoptosis in PC12 cells in a dose-dependent manner; (2) the preconditioning with 10 μmol Lâ1 or 20 μmol Lâ1 H2O2 can significantly protect PC12 cells against apoptosis induced by 50 or 100 μmol Lâ1 H2O2, low (5 μmol Lâ1) and higher (30 μmol Lâ1) concentrations of H2O2 had no cytoprotections; (3) high concentration (100 μmol Lâ1) of H2O2 reduced MMP and expression of Bcl-2, and increased ROS level, but these effects were blocked by preconditioning with 10 μmol Lâ1 H2O2; (4) the preconditioning with 10 μmol Lâ1 H2O2 induced overexpression of Bcl-2. These results suggested that the preconditioning with low dose of H2O2 could protect the oxidative stress-induced PC12 cells apoptosis not only by preventing the reduction of MMP and expression of Bcl-2 as well as increase in ROS level, but also through overexpression of Bcl-2. It was indicated that overexpression of Bcl-2 may play a key role in the cytoprotection induced by preconditioning with low dose of H2O2 in PC12 cells.
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Neuroscience
Neuroscience (General)
Authors
Xiao-Qing Tang, Jian-Qiang Feng, Jin Chen, Pei-Xi Chen, Jun-Li Zhi, Yu Cui, Rui-Xian Guo, Hui-Min Yu,