Postsynaptic potentials of tectal neurons evoked by electrical stimulation of the pretectal nuclei in bullfrogs (Rana catesbeiana)

Postsynaptic responses of the tectal cells to electrical stimulation of pretectal (Lpd/P) nuclei were intracellularly recorded in the bullfrog (Rana catesbeiana). The pretectal stimulation elicited mainly two types of responses in the ipsilateral tectum: an EPSP followed by an IPSP and a pure IPSP. The latter predominates in the tectal cells responding to ipsilateral pretectal stimulation. In a few cells, biphasic hyperpolarization appeared under stronger stimulus intensities. Only one type of response was found in the contralateral tectum, a pure IPSP. The antidromically invaded tecto-pretectal projecting cells were recorded in both tecta, which revealed reciprocal connections between the tectum and particular pretectal nuclei. This paper demonstrates the synaptic nature underlying pretectotectal information transfer. EPSPs with short latencies were concluded to be monosynaptic. Most IPSPs were generated through polysynaptic paths, but monosynaptic IPSPs were also recorded in both optic tecta. Nearly 98% of impaled tectal cells (except for intra-axonally recorded and antidromically invaded cells) showed inhibitory responses to pretectal stimulation. The results provide strong evidence that pretectal cells broadly inhibit tectal neurons as suggested by behavioral and extracellular recording studies.