Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9416529 | Brain Research | 2005 | 11 Pages |
Abstract
Molecular events associated with mnemonic processes and neuronal plasticity are postulated to result in functional changes in synaptic structure. One possible site is the post-synaptic density, where activity-dependent changes modulate signal transduction cascades. In this report, we detail spatial-temporal changes for phosphodiesterase 4B (PDE4B) proteins and their substrate cAMP within three neuronal fractions during early and late long-term potentiation (LTP). The cAMP-dependent protein kinase A cascade - which can be regulated by distinct PDE4B activity - is required for mnemonic processes as well as mechanisms of neuronal plasticity, such as those during the maintenance or late-LTP. Fluorescence in situ hybridization studies (FISH) identified no translocation of PDE4B3 from the soma after late-LTP induction indicating a subtle, local control of PDE4B activity. Protein changes were detected within the PSD-enriched fraction. From these results, we conclude that either the changes in PDE4B are due to modulation of pre-existing mRNA, or that the protein is specifically translocated to activated synaptic structures. Furthermore, we report late changes in cAMP levels in the somato-dendritic fraction and discuss this result with the increased PDE4B1/3 doublet in the PSD-enriched fraction.
Keywords
PAGEACDTBSTPKCHFSLFSN-methyl-d-aspartateNMDApKaTETPDE4PDEpoly-acrylamide gel electrophoresiscAMP-response-element-binding proteinPBSCREBcAMPSTPEnzyme-immunoassayactinomycin DEIAlow-frequency stimulationhigh-frequency stimulationlong-term potentiationLong-term potentiation: physiologyLTPmemory formationExcitable membranes and synaptic transmissionPhosphate buffered salinePhosphodiesterasefluorescence in situ hybridizationFishPostsynaptic mechanismsHippocampusShort-term potentiationProtein kinase CcAMP-dependent protein kinaseNeuronal plasticity
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Authors
T. Ahmed, J.U. Frey,