Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9416653 | Brain Research | 2005 | 7 Pages |
Abstract
The preoptic/anterior hypothalamus (POA) is one of the major brain regions where cytokines and their related mediators (i.e., prostaglandins) exert diverse actions. In the present study, the modulatory effects of prostaglandin E2 (PGE2) on the glutamate-induced membrane currents were examined using perforated-patch clamp method in rat POA neurons that had been mechanically dissociated by vibration without enzyme treatment. Application of glutamate through U-tube induced a slow outward current following fast inward ionotroic current at a holding membrane potential of â30 mV. The slow outward current was also induced by N-methyl-d-aspartate (NMDA), accompanied by an increased membrane conductance, and inhibited by perfusion with Ca2+-free solution, tetraethylammonium chloride (TEA), and apamin, suggesting a Ca2+-dependent K+ current (KCa) activated by Ca2+ entry through NMDA channels. Perfusion with PGE2 at 0.1-10 μM, a principal mediator of fever and neuroendocrine control at the POA, did not produce apparent current by itself, but selectively potentiated the glutamate- or NMDA-induced KCa without affecting inward currents. The KCa induced by activation of NMDA receptors may serve as a feedback mechanism and the modulatory effects of PGE2 on the KCa may have an important physiological significance.
Keywords
Related Topics
Life Sciences
Neuroscience
Neuroscience (General)
Authors
Toshihiko Katafuchi, Shumin Duan, Sachiko Take, Megumu Yoshimura,