Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9416705 | Brain Research | 2005 | 9 Pages |
Abstract
Ischemia/hypoxia (I/H) causes severe perinatal brain disorders such as cerebral palsy. The neonatal brain possesses much plasticity, and to enhance new cell production would be an innovative means of therapy for such disorders. In order to elucidate the dynamic changes of neural progenitor cells in the neonatal brain after ischemia, we investigated new cells production in the subventricular zone and subsequent migration of these cells to the injured area. Newly produced cells were confirmed by incorporation of bromodeoxyuridine (BrdU), and attempt for differentiation was investigated by immunohistochemistry for molecular markers of each cellular lineage. In the sham-control brain, there were many BrdU-labeled cells which gradually decreased as the animal becomes older. Many of these cells were oligodendroglial progenitor or microglial cells. Although there were only few neuronal cells labeled for BrdU in the sham-control, they dramatically increased after I/H. They were located at just beneath the subventricular zone where the progenitor cells reside and to the injured area, indicating that newly produced cells migrated to the infarct region and differentiated into neuronal precursor cells in order to compensate the lost neural cells. We found that BrdU-labeled astroglial, oligodendroglial progenitor, and microglial cells were also increased after I/H, suggesting that they also play active roles in recovery. Progenitor cells may have potential for treating perinatal brain disorders.
Keywords
PBSSVZIBA1DcxGFAPMCADevelopmental disordersDisorders of the nervous systemstandard deviationIschemiaIschemia/hypoxiaBrdUbromodeoxyuridinedoublecortinprogenitor cellmiddle cerebral arteryPhosphate buffered salinesubventricular zoneMigrationRationized calcium-binding adapter molecule-1NeonateHypoxiaGlial fibrillary acidic protein
Related Topics
Life Sciences
Neuroscience
Neuroscience (General)
Authors
Takeshi Hayashi, Masanori Iwai, Tomoaki Ikeda, Guang Jin, Kentaro Deguchi, Shoko Nagotani, Hanzhe Zhang, Yoshihide Sehara, Isao Nagano, Mikio Shoji, Tsuyomu Ikenoue, Koji Abe,