Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9424252 | Journal of Neuroscience Methods | 2005 | 9 Pages |
Abstract
Neuroinflammation is one of the events occurring after acute brain injuries. The aim of the present report was to characterize a rat model to study acute neuroinflammation on the histopathological, biochemical and functional outcomes. Lipopolysaccharide (LPS), known as a strong immunostimulant, was directly injected into the hippocampus. The spatiotemporal evolution of inducible NOS (iNOS) and cell death was studied from 6Â h to 7 days. A perfect time course correlation was observed between iNOS immunoreactivity and iNOS activity showing an acute, expansive and transient iNOS induction in the hippocampus with a peak at 24Â h. It was associated with a marked increase in NO metabolite (NOx) levels, and a high level of myeloperoxidase (MPO) activity. This inflammation precedes a massive cellular loss including at least neurons and astrocytes, and a drop of constitutive NOS activity, restrictive to the ipsilateral hippocampus from 48Â h after LPS injection. Moreover, sensorimotor function impairment occurred from 24Â h to 7 days with a maximum at 24Â h post-LPS injection. Therefore, we characterized an in vivo model of acute neuroinflammation and neurodegeneration, in relation with a neurological deficit, which may be a powerful tool for mechanistic studies and for further evaluation of the potential neuroprotective agents.
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Authors
Andrea Ambrosini, Gaëlle Louin, Nicole Croci, Michel Plotkine, Mehrnaz Jafarian-Tehrani,