Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9424361 | Journal of Neuroscience Methods | 2005 | 4 Pages |
Abstract
Oxidative stress is a pivotal factor in neuronal degeneration. A simple method to quantify oxidative damage in culture and in situ is therefore important for studies of neurodegeneration. We present herein modifications of the standard assay for thiobarbituric acid-reactive substances (TBARs) for analyses of both cell cultures and brain tissue homogenates. Since the TBAR assay measures end-point oxidative damage, it is useful to assess the overall impact of oxidative stress-inducing and neuroprotective agents; interpretation is not potentially confounded by the presence or absence of transient products of oxidative damage.
Keywords
Related Topics
Life Sciences
Neuroscience
Neuroscience (General)
Authors
Maria Dawn-Linsley, Fatma J. Ekinci, Daniela Ortiz, Eugene Rogers, Thomas B. Shea,