Pulser: user-friendly, graphical user-interface based software for controlling stimuli during data acquisition with Spike2 for Windows

This paper describes software that runs in the Spike2 for Windows environment and provides a versatile tool for generating stimuli during data acquisition from the 1401 family of interfaces (CED, UK). A graphical user interface (GUI) is used to provide dynamic control of stimulus timing. Both single stimuli and trains of stimuli can be generated. The pulse generation routines make use of programmable variables within the interface and allow these to be rapidly changed during an experiment. The routines therefore provide the ease-of-use associated with external, stand-alone pulse generators. Complex stimulus protocols can be loaded from an external text file and facilities are included to create these files through the GUI. The software consists of a Spike2 script that runs in the host PC, and accompanying routines written in the 1401 sequencer control code, that run in the 1401 interface. Handshaking between the PC and the interface card are built into the routines and provides for full integration of sampling, analysis and stimulus generation during an experiment. Control of the 1401 digital-to-analogue converters is also provided; this allows control of stimulus amplitude as well as timing and also provides a sample-hold feature that may be used to remove DC offsets and drift from recorded data.