Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9424390 | Journal of Neuroscience Methods | 2005 | 5 Pages |
Abstract
Brain serotonin 5-HT1A and 5-HT2A receptors have been implicated in both normal and pathological behavior, and in the action of anxiolytic and antidepressant drugs. In this study, detailed description and verification of a new RT-PCR technique to quantify the number of copies of 5-HT1A and 5-HT2A receptor mRNAs in the brain is presented. The number of copies of β-actin and 5-HT1A or 5-HT2A receptor mRNAs in rat brain samples was evaluated with respect to the genomic DNA solution as the external exogenous standard. The expression of 5-HT receptors was calculated as the number of receptor mRNA copies per 100 copies of corresponding β-actin mRNA. This presented technique is reliable, simple and can be easily set up in any neurobiological laboratory.
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Authors
Alexander V. Kulikov, Vladimir S. Naumenko, Irina P. Voronova, Maria A. Tikhonova, Nina K. Popova,