Article ID Journal Published Year Pages File Type
9425446 Neuroscience 2005 13 Pages PDF
Abstract
A three-dimensional (3D) culture of newborn rat utricles using an extracellular matrix sustaining 3D cellular growth was developed during 3, 6, or 10 days in vitro (DIV). Using morphological and electrophysiological techniques, we describe the de novo formation of a cyst. It was composed of the sensory epithelium and non-sensory cells-canalar, dark and intermediate cells-that polarized so that their apical surface faced its lumen. During the time of culture, the utricular potential (UP) was steady (−1.1±5.0 mV) in oxygenated condition, while in anoxia, the UP significantly decreased to −8.4±1.0 mV at 8 DIV. Over the same period, the K+ concentration in the cyst increased up to 86.1±33.9 mM (versus 5.6±1.5 mM in the bath). These observations indicated that the mechanisms generating the UP and the K-secretory activity were functional at this stage. Concomitantly, the hair cells acquired mature and functional properties: the type 1 and type 2 phenotypes, a mean resting membrane potential of −68.1±4.6 mV and typical electrophysiological responses. This preparation provides a powerful means to simultaneous access the hair cells and their endolymphatic compartment, with the possibility to use multi-technical approaches to investigate their interdependent relationships.
Related Topics
Life Sciences Neuroscience Neuroscience (General)
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