Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9425446 | Neuroscience | 2005 | 13 Pages |
Abstract
A three-dimensional (3D) culture of newborn rat utricles using an extracellular matrix sustaining 3D cellular growth was developed during 3, 6, or 10 days in vitro (DIV). Using morphological and electrophysiological techniques, we describe the de novo formation of a cyst. It was composed of the sensory epithelium and non-sensory cells-canalar, dark and intermediate cells-that polarized so that their apical surface faced its lumen. During the time of culture, the utricular potential (UP) was steady (â1.1±5.0 mV) in oxygenated condition, while in anoxia, the UP significantly decreased to â8.4±1.0 mV at 8 DIV. Over the same period, the K+ concentration in the cyst increased up to 86.1±33.9 mM (versus 5.6±1.5 mM in the bath). These observations indicated that the mechanisms generating the UP and the K-secretory activity were functional at this stage. Concomitantly, the hair cells acquired mature and functional properties: the type 1 and type 2 phenotypes, a mean resting membrane potential of â68.1±4.6 mV and typical electrophysiological responses. This preparation provides a powerful means to simultaneous access the hair cells and their endolymphatic compartment, with the possibility to use multi-technical approaches to investigate their interdependent relationships.
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Authors
S. Gaboyard, C. Chabbert, C. Travo, F. Bancel, J. Lehouelleur, D. Yamauchi, D.C. Marcus, A. Sans,