Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9425732 | Neuroscience | 2005 | 10 Pages |
Abstract
Mitogen activated protein kinases such as phosphorylated extracellular signal-regulated kinase-1 and -2 (pERK 1/2) have been recently demonstrated to play an important role in somatic nociception and hyperalgesia. In the present study we examined whether pERK 1/2 is involved in the response of sensory neurons to a noxious visceral stimulation, in particular, of the gastric mucosa. After induction of gastric injury by oral administration of 0.5M HCl pERK 1/2 expression was determined by Western blotting of caudal thoracic dorsal root ganglia and by immunohistochemistry in stomach-innervating dorsal root ganglion neurons which were retrogradely labeled with True Blue. The content of pERK 1/2 remained unchanged in dorsal root ganglia until 2 h post-HCl, however, was found elevated 4 (â¼80%) and 6 h (â¼100%) after HCl administration. True Blue-labeled pERK 1/2-immunoreactive neurons were likewise increased 6 h post-HCl (204%) and were mainly of small size (20-40μm) and negative for neurofilament 200 (â¼76%). The majority of these cells also expressed the nociceptive transient receptor potential vanilloid receptor 1 (â¼70%). The gastric mucosa was simultaneously examined for lesion formation showing highest percentage of damage 6 h post-HCl. Application of a N-methyl-d-aspartate receptor antagonist (MK-801; 100μg/kg s.c.) significantly reduced HCl-induced pERK 1/2 expression and mucosal lesions 6 h post-HCl. Activation of the extracellular signal-regulated kinase-1 and -2 signaling cascade indicates that visceral primary afferents may sensitize after gastric noxious stimulation involving N-methyl-d-aspartate receptors. The extracellular signal-regulated kinase-1 and -2 pathway therefore may not only be of importance for somatic but also for visceral nociception.
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Authors
R. Schicho, I. Liebmann, I.T. Lippe,