Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9434354 | Neuroscience Research | 2005 | 7 Pages |
Abstract
Mammalian cells that have been committed to a certain cell lineage cannot be directed to other lineages. However, some astrocytes in the mammalian brains have been reported to represent plasticity to redirect to other cell lineages. We found that mouse hippocampal astrocytes cultured in aggregate forms of “astrosphere”, redirected to MAP2-positive immature neurons. In astrospheres, basic HLH factors positively regulating neuronal differentiation were up-regulated and Id3 inhibiting basic HLH factors was down-regulated. Ectopic Id3 induction repressed redirection of astrocytes to a neuronal lineage, suggesting that astrosphere formation induced plasticity of astrocytes by changing the gene expression patterns.
Keywords
EGFG3PDHNGNMAP2FCSRT-PCRHLHbFGFGFPGFAPNSCAstrocyteDIVDevelopmentday in vitrofetal calf serumCell fateNeural stem cellshelix–loop–helixepidermal growth factorbasic fibroblast growth factorBMPNeuronneurogeninRedirectionHEMAreverse transcription-polymerase chain reactionGlial fibrillary acidic proteingreen fluorescent proteinmicrotubule-associated protein 2Bone morphogenetic proteinpoly(2-hydroxyethyl methacrylate)glyceraldehyde-3-phosphate dehydrogenase
Related Topics
Life Sciences
Neuroscience
Neuroscience (General)
Authors
Makoto Yanagisawa, Kinichi Nakashima, Wataru Ochiai, Takumi Takizawa, Takao Setoguchi, Atsumi Uemura, Makiko Takizawa, Ikuo Nobuhisa, Tetsuya Taga,