Searching for hypothetical proteins: Theory and practice based upon original data and literature

Article ID	Journal	Published Year	Pages	File Type
9435098	Progress in Neurobiology	2005	38 Pages	PDF

Abstract

We here provide information about hypothetical proteins in terms of protein chemical analysis, independent of antibody availability and specificity and bioinformatic handling to contribute to the extension/completion of protein databases and include original work on HP in the brain to illustrate the processes of HP identification and functional assignment.

Keywords

Pfam OGP PMF 2-DE TFA CoG ORF TOF Q-TOF DIP LRR CATH NNA Supervised locally linear embedding UPF FSSP ANN ORFans PBS Clusters of Orthologous Groups of proteins nuclear magnetic resonance 3-[(3-cholamidopropyl)dimethylammonio]-1-propane-sulfonate CDD MS/MS PSI-BLAST Simple modular architecture research tool Basic Local Alignment Search Tool EDTA Ethylenediaminetetraacetic acid Peptide mass fingerprint Trifluoroacetic acid ELM two-dimensional gel electrophoresis Neural network algorithm Blast Biomolecular Interaction Network Database bind grand average of hydropathy Bioinformatics NMR Leucine rich repeats time of flight quadrupole time-of-flight artificial neural networks Structural Classification of Proteins protein structure classification Mass spectrometry Tandem mass spectrometry Open reading frames Phosphate buffered saline Matrix-assisted laser desorption/ionisation SVM Support vector machine MALDI Medulloblastoma SCOP SMART conserved domain database Database of Interacting Proteins Hypothetical protein Signal peptide Prediction CHAPS liquid chromatography GRAVY