| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9436536 | Hearing Research | 2005 | 8 Pages |
Abstract
In conclusion, we showed that the prestin mRNA levels during in vitro development increase and form an apical-basal gradient within 2 days in culture, similar to the postnatal in vivo development. Hypoxia and ischemia result in a decrease of the prestin mRNA level in parallel with OHC loss. The prestin mRNA level can therefore be used as marker of damage to or loss of OHCs.
Keywords
tris-acetate-EDTAdNTPcRNAQ-RT-PCROHCFCSDEPCPAGEcopy DNAPolyacrylamide electrophoresisDTTcopy RNABSAcDNADMEM/F12bovine serum albuminIschemiaTAEbase pairsCochleadiethylpyrocarbonatedithiothreitolpostnatal dayfetal calf serumouter hair cellDulbecco’s modified eagle’s mediumHypoxiaquantitative reverse transcription polymerase chain reactionpolymerase chain reactionPCR
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Authors
Johann Gross, Astrid Machulik, Nyamaa Amarjargal, Julia Fuchs, Birgit Mazurek,