Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9439940 | Research in Microbiology | 2005 | 7 Pages |
Abstract
Two forms of acetyl-CoA synthetase (ACS1 and ACS2) have been detected in Phycomyces blakesleeanus. ACS1, encoded by the gene facA, was induced by acetate and repressed by glucose at the transcriptional level. ACS2, not encoded by the gene facA, was detected as a response to carbon starvation both in the wild type and in an facAâ mutant. Both enzymes were purified and characterized. They can use acetate and propionate as substrates. ACS2 is a much more stable enzyme than ACS1. After 60 min incubation at 55â°C, ACS2 retained 50% of its activity whereas ACS1 only retained 3%. The optimum temperature was 50â°C for ACS2 and 30â°C for ACS1.
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Authors
Sergio De Cima, Javier Rúa, Eusebio Perdiguero, Pilar del Valle, Félix Busto, Alberto Baroja-Mazo, Dolores de Arriaga,