Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9442588 | Experimental Parasitology | 2005 | 9 Pages |
Abstract
The elucidation of factors inducing the growth of Plasmodium falciparum can provide critical information about the developmental mechanisms of this parasite and open the way to search for novel targets for malaria chemotherapy. The ability of components of a growth-promoting factor derived from bovine serum and various related substances to sustain growth of P. falciparum was characterized. A simple total lipid fraction (GFS-C) containing non-esterified fatty acids (NEFAs) as essential factors was noted to promote the parasite's growth. Various proteins from a variety of animals were tested, indicating the importance not only of GFS-C, but also of specific proteins, such as bovine and human albumin, in the parasite growth. Several combinations of the NEFAs tested sustained low parasite growth. Among various phospholipids and lysophospholipids tested, lysophosphatidylcholine containing C-18 unsaturated fatty acids was found to sustain the complete development of the parasite in the presence of bovine albumin. Several other lysophospholipids can partially support growth of P. falciparum.
Keywords
CDPKsLPEC18:3PKCLPARBCsNEFAMGSGFSDGsCHOLlysophosphatidyl ethanolamineBSAFC18:0S-1-PPTKpLDHC16:1cRPMIcis-9-octadecenoic acidALBHEPESSPCLPILPCPLSLPSFBSRed blood cellsC16:0C18:1C18:2CDKsMAPKAlbuminsphingomyelinSphingosylphosphorylcholinesphingosine 1-phosphatesphingosineOctadecanoic acidlysophosphatidic acidHexadecanoic acidNon-esterified fatty acidstandard deviationparasite lactate dehydrogenaseANOVADiglyceridesBasal mediumHuman serumfetal bovine serumPhosphatidyl inositolphosphatidyl ethanolaminephosphatidyl serinephosphatidylcholinePhospholipidsLysophosphatidylcholineLysophospholipidsMEKmonoglyceridesPAFProtein tyrosine kinaseProtein kinase Cmitogen-activated protein kinasecyclin-dependent protein kinasescalcium-dependent protein kinasescholesterol
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Life Sciences
Immunology and Microbiology
Parasitology
Authors
Hiroko Asahi, Tamotsu Kanazawa, Nakami Hirayama, Yousei Kajihara,