Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9442615 | Experimental Parasitology | 2005 | 6 Pages |
Abstract
Despite of our knowledge of genetic make up of schistosomes, a number of genes have not been characterized largely due to lack of effective transformation protocols. Here we present electroporation as a strategy for effective introduction of plasmids DNA into schistosomula and adults. Using plasmids of pEGFP-C1 as an expression vector, we first verified that the CMV promoter could direct EGFP to express in primary culture cells from Schistosoma japonicum. Subsequently, the plasmids were introduced into schistosomula and adults by electroporation and EGFP expression was demonstrated using molecular and microscopical methods. Our findings indicate that electroporation is an effective method for transformation of S. japonicum.
Keywords
PBSFBSDABdiaminobenzidine tetrahydrochlorideeGFPRT-PCRSDS–PAGEEDTAethylene diamine tetraacetic acidsodium dodecyl sulfate–polyacrylamide gel electrophoresisElectroporationTransgenic parasiteTransformationfetal bovine serumSchistosomeSchistosoma japonicumPhosphate-buffered salinereverse transcription-polymerase chain reactionenhanced green fluorescent protein
Related Topics
Life Sciences
Immunology and Microbiology
Parasitology
Authors
Xiao-Song Yuan, Ji-Long Shen, Xue-Long Wang, Xue-Song Wu, De-Pei Liu, Hui-Fen Dong, Ming-Seng Jiang,