| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9442761 | Experimental Parasitology | 2005 | 4 Pages |
Abstract
Trypanosoma vivax is the principal etiological agent of bovine trypanosomosis, a widely disseminated disease in tropical and subtropical regions. Here, we present a simple and reproducible method for the purification of T. vivax from experimentally infected and immunosuppressed sheep, using an isopycnic Percoll gradient, followed by DEAE-cellulose chromatography, with an estimated yield of 11-15%. This method could be used for the purification of T. vivax geographical isolates from various locations and from different natural hosts.
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Authors
L.E. González, J.A. GarcÃa, C. Núñez, T.M. Perrone, B. González-Baradat, M.I. Gonzatti, A. Reyna-Bello,