Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9451901 | Chemosphere | 2005 | 5 Pages |
Abstract
Due to the numerous biotechnological applications of laccase enzyme, it is essential to know the influence of different agents usually present in the natural environment on its enzymatic action, especially for in situ treatment technologies. In the present work, a simple and rapid method to determine the inhibitory or inducer effect of different compounds on laccase activity was developed. The compounds tested were copper-chelating agents and heavy metals. It was found that using syringaldazine as a substrate, all copper-chelating agents (except EDTA) highly inhibited laccase activity (around 100%) at an inhibitor concentration lower than 20Â mM. Moreover, 40% of inhibition, which was detected at a concentration of 20Â mM for both Cd2+ and Cu2+ increased with concentration until nearly complete inhibition at 80Â mM.
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Authors
M. Lorenzo, D. Moldes, S. RodrıÌguez Couto, MaA. Sanromán,