Article ID Journal Published Year Pages File Type
9557329 Phytochemistry 2005 8 Pages PDF
Abstract
Potato isoapyrases from Desirée (low ATPase/ADPase ratio) and Pimpernel (high ATPase/ADPase ratio) show broad specificities towards dNTP and fluorescent nucleotides. Pimpernel enzyme shows higher kcat towards all triphosphate derivatives and lower proteolytic susceptibility as compared with Desirée. Unlike animal apyrases, labeling with azido-nucleotides and FSBA was not achieved with these plant enzymes.
Related Topics
Physical Sciences and Engineering Chemistry Organic Chemistry
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