Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9557329 | Phytochemistry | 2005 | 8 Pages |
Abstract
Potato isoapyrases from Desirée (low ATPase/ADPase ratio) and Pimpernel (high ATPase/ADPase ratio) show broad specificities towards dNTP and fluorescent nucleotides. Pimpernel enzyme shows higher kcat towards all triphosphate derivatives and lower proteolytic susceptibility as compared with Desirée. Unlike animal apyrases, labeling with azido-nucleotides and FSBA was not achieved with these plant enzymes.
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Organic Chemistry
Authors
A.M. Kettlun, V. Espinosa, L GarcÃa, M.A. Valenzuela,