Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9562749 | Reactive and Functional Polymers | 2005 | 6 Pages |
Abstract
A molecularly imprinted polypyrrole (MIPPy) film was synthesized on the Spreeta sensor, a miniaturized surface plasmon resonance device, for detection of ochratoxin A (OTA). The MIPPy was electrochemically polymerized on the sensor surface from a solution of pyrrole and OTA in ethanol/water (1:9 v/v). The film growth was monitored in situ by an increasing SPR angle. Binding properties of the MIPPy film were investigated by loading OTA standard solutions into the integrated 20-μL flow cell. After 300 s, nonlinear regression was used to determine the maximum binding signal (Î300). Spreeta results showed that Î300 was measurable for OTA concentrations down to 0.05 ppm. By pulsed elution, 1% acetic acid in methanol/water (1:9 v/v) was found to be efficient for regeneration of the MIPPy film surface. Interference by the matrices of wheat and wine extracts was evaluated. No significant binding of the wheat extract with MIPPy was observed when acetonitrile/water (1:1 v/v) was used as the mobile phase.
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Jorn C.C. Yu, Edward P.C. Lai,