Article ID Journal Published Year Pages File Type
9577942 Chemical Physics Letters 2005 5 Pages PDF
Abstract
We study the fluorescence patterns produced by single CdSe nanocrystals in defocused microscopy. The patterns are directly related to the structure of the nanocrystal emission dipole and their analysis provides a full determination of the three-dimensional orientation of the nanoparticles. In the case of a high numerical aperture objective, we show that the contour intensities of the defocused patterns can be fitted by a simple analytical formula. Finally, we demonstrate that defocused imaging can be used to track both the position and the orientation of single CdSe nanocrystals coupled to membrane receptors in live cells.
Related Topics
Physical Sciences and Engineering Chemistry Physical and Theoretical Chemistry
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