Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9694054 | Thermochimica Acta | 2005 | 5 Pages |
Abstract
This investigation presents measurements of isothermal ssDNA oligomer hybridization enthalpy at various DNA length, GC contents, temperatures, and salt concentrations by isothermal titration calorimetry (ITC) at temperatures below the melting temperature of the DNA. The study aimed to reveal the hybridization mechanism of ssDNA for gene chip applications. The role of hydrogen bonds between complementary bases, the stacking forces between bases, the electrostatic repulsion between strands, the dehydration and conformational rearrangement of ssDNA in hybridization are estimated from the interaction enthalpy.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Fluid Flow and Transfer Processes
Authors
An-Cheng Liu, Liang-Yu Chen, Chung-Fan Chiou, Hung-Ju Richard Su, Yu-Chia Chan, Pei-Hsuan Tasi, Shean-Jen Chen, Wen-Yih Chen,