Lipophilic conjugates of methotrexate with glucosyl-lipoamino acids: calorimetric study of the interaction with a biomembrane model

On the contrary, by incubating the conjugates with pre-formed empty DMPC liposomes at 37 °C, these derivatives were unable to penetrate the bilayers, most probably because of the presence of the two sugar residues. This behavior was correlated with the low biological activity observed in vitro for these conjugates, with respect to a corresponding series of MTX-LAA conjugates.