Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9748506 | Journal of Chromatography A | 2005 | 5 Pages |
Abstract
Aesculetin is the product of the hydrolysis reaction of aesculin. A high sensitivity and good repeatability method based on capillary electrophoresis with amperometric detection (CE-AD) was developed for simultaneous determination of aesculin and aesculetin in the hydrolysate of aesculin. Under the optimum condition: 10 mmol/L KH2PO4-5 mmol/L Na2B4O7 (pH 6.0) buffer, separation at 18 kV and +900 mV (versus Ag/AgCl) as the detection potential, the hydrolysis rate constants of aesculin hydrolysis at 25, 30, 35, 40 and 45 °C in 0.1 mol/L KOH were obtained as 1.45 Ã 10â2 minâ1, 2.01 Ã 10â2 minâ1, 2.93 Ã 10â2 minâ1, 3.76 Ã 10â2 minâ1 and 5.05 Ã 10â2 minâ1, respectively. It was calculated that the activation energy for aesculin hydrolysis was 49.4 kJ/mol.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Lan Zhang, Ping Tong, Guonan Chen,