Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9748621 | Journal of Chromatography A | 2005 | 4 Pages |
Abstract
Contactless conductivity detection was employed for the detection of the enantiomers of 1-phenylethylamine and 1-cyclohexylethylamine which were separated in capillary electrophoresis with unprecedented high resolutions Rs of 2.3 and 3.3, respectively, by using a combination of dimethyl-β-cyclodextrin and the chiral crown ether 18C6H4 as chiral selectors in a citric acid buffer of pH 2.4. The conductivity measurement enabled the direct detection, i.e. without having to derivatize or resort to indirect methods, of all species including the non-UV-absorbing enantiomers of cyclohexylamine. Detection limits of 0.5 μM were achieved and the determination of enantiomeric ratios of up to 99:1 was found possible.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xiao Yang Gong, Peter C. Hauser,