Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9748633 | Journal of Chromatography A | 2005 | 10 Pages |
Abstract
Alginates comprised of essentially alternating units of mannuronic (M) acid-guluronic (G) acid (MG-alginate), and G-blocks isolated from a seaweed where subjected to partial acid hydrolysis at pH 3.5 The chain-length distribution of oligosaccharides in the hydrolysate were investigated by statistical analysis after their separation with high-performance anion-exchange chromatography and pulsed amperometric detection (HPAEC-PAD). Simulated depolymerisation of the MG-alginate provided an estimate of the ratio between two acid hydrolysis rate constants (p = 8.3 ± 1) and the average distribution of the MM linkages in the original sample of polysaccharide chains. In conclusion, we found HPAEC-PAD together with statistical analysis was a useful method to investigate the fine structure and some properties of binary polysaccharides.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Simon Ballance, Synnøve Holtan, Olav Andreas Aarstad, Pawel Sikorski, Gudmund Skjåk-Bræk, Bjørn E. Christensen,