Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9748647 | Journal of Chromatography A | 2005 | 8 Pages |
Abstract
Glycoproteins from sugarcane stalks have been isolated from plants field-grown by size-exclusion chromatography. Some of these glycoproteins, previously labelled with fluorescein isothiocyanate, are able to bind to the cell wall of the sugarcane endophyte, N2-fixing Gluconacetobacter diazotrophicus, and largely removed after washing the bacterial cells with sucrose. This implies that sugarcane glycoproteins use β-(1 â 2)-fructofuranosyl fructose domains in their glycosidic moiety to bind to specific receptors in the bacterial cell walls. These receptors have been isolated by affinity chromatography on a sugarcane glycoprotein-agarose matrix, desorbed with sucrose and characterized by sodium dodecyl sulfate polyacrylamide gel electrophresisand capillary electrophoresis (CE).
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Y. Blanco, M. Arroyo, M.E. Legaz, C. Vicente,