| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9748658 | Journal of Chromatography A | 2005 | 9 Pages |
Abstract
Lipophilicity was evaluated using a novel RP-HPLC stationary phase (Discovery-RP-Amide-C16) with and without 1-octanol added to the mobile phase. A set of 46 drugs and flavonoids characterized by a broad structural diversity and a wide log Poct range (â0.69 to 5.70) was selected for this study. This set consists of neutral solutes and solutes with acidic or ampholytic functionalities which were maintained neutral at pH 2.5 or 4. In our conditions, the addition of 1-octanol in the mobile phase proved a key factor to derive a lipophilicity index log kw highly correlated with log Poct for all investigated solutes. 1-Octanol improved the correlation between log Poct and log kw mainly by influencing the retention behavior of the solutes with log Poct values below +3. This study brings additional evidence that under proper experimental conditions of stationary and mobile phases, RP-HPLC is a very useful method to obtain log Poct values.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xiangli Liu, Hideji Tanaka, Aiko Yamauchi, Bernard Testa, Hiroshi Chuman,